|
| Status |
Public on Aug 02, 2012 |
| Title |
day 30, CEA424-Tag mouse 2 |
| Sample type |
RNA |
| |
|
| Source name |
antrum/stomach
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57Bl/6
age: 30 d
tissue: stomach antrum tumor with normal tissue
genotype: CEA424-SV40 -T antigen transgenic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Nucleospin extraction kit according to the manufacturer´s protocol ((Machery&Nagel, Düren, Germany). Quality control was performed with denaturing gel electrophoresis and UV measurement.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Illumina® TotalPrep™ RNA Amplification Kit (Applied Biosystems, Darmstadt, Germany).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
tumor with normal tissue
|
| Data processing |
The data were normalised using vsn normalisation using R Statistical language and packages from the Bioconductor project
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
Aug 02, 2012 |
| Contact name |
Joachim L Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
University of Bonn
|
| Department |
LIMES Institute
|
| Lab |
Genomics & Immunoregulation
|
| Street address |
Carl-Troll-Str. 31
|
| City |
Bonn |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4055 |
| Series (2) |
| GSE27711 |
Gene expression profiles of spontaneous gastric tumors in the antrum of the stomach of 30,60, and 90 day old CEA424-SV40 -T antigen transgenic mice. |
| GSE27712 |
GC424 tumor cells and gastric tumors |
|
