|
| Status |
Public on Jun 07, 2023 |
| Title |
STZ3 |
| Sample type |
SRA |
| |
|
| Source name |
NRK-52E
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: NRK-52E
cell type: Kidney epithelial cell
genotype: WT
treatment: STZ
|
| Treatment protocol |
STZ groups were treated with 10 mM of STZ for 24 h before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cortex of kidneys or NRK-52E cells using TRIzol (Life Technologies, Carlsbad, CA) and Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA)
Library preparation was performed using a TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced reads were mapped to the rat reference genome sequences (Rnor6) using STAR v 2.7.10a (https://github.com/alexdobin/STAR/) and uniquely mapped reads were counted by featureCounts function in the Subread package (https://subread.sourceforge.net).
Assembly: Rnor6
Supplementary files format and content: tab-delimited text files include gene counts for each Sample
|
| |
|
| Submission date |
Oct 12, 2022 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Tetsuro Kusaba |
| Organization name |
Graduate School of Medical Science, Kyoto Prefectural University of Medicine
|
| Department |
Department of Nephrology
|
| Street address |
465 Kajii-cho, Kamigyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
6028566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE215337 |
Streptozotocin induces renal proximal tubular injury through p53 signaling activation |
|
| Relations |
| BioSample |
SAMN31257449 |
| SRA |
SRX17865248 |
