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Sample GSM636751 Query DataSets for GSM636751
Status Public on Dec 09, 2010
Title colon_cornoil_10wk_rep3 [136.3]
Sample type RNA
 
Source name colonic mucosa, fed corn oil, terminated 10 wk after carcinogen injection
Organism Rattus norvegicus
Characteristics diet: corn oil
time point: terminated 10 wk after carcinogen injection
strain: Sprague Dawley
gender: Weanling male
tissue: colon
Treatment protocol Rats were treated with the carcinogen azoxymethane at 15 mg/kg body weight and terminated 12 h later or were given a second injection and terminated 9 wk later.
Growth protocol Rats were fed diets that differed in type of fat (corn oil or fish oil or olive oil).
Extracted molecule total RNA
Extraction protocol Ambion Totally RNA kit followed by DNAFree treatment.
Label biotin, Alexa-647 Streptavidin
Label protocol Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Data processing Median normalized values within each array as calculated in Microsoft Excel version 2003
 
Submission date Dec 08, 2010
Last update date Dec 09, 2010
Contact name Jennifer Goldsby
E-mail(s) jsgoldsby@tamu.edu
Phone 979-845-3908
Organization name Texas A&M University
Department Nutrition and Food Sciences
Street address 112 Cater-Mattil
City College Station
State/province TX
ZIP/Postal code 77845
Country USA
 
Platform ID GPL2890
Series (1)
GSE25912 Classification of diet modified gene signatures at the colon cancer initiation and progression stages

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
109 1.588712221
110 1.320902941
111 1.913367013
112 1.237393154
113 2.710810202
114 0.979588934
115 0.919339184
116 1.568039786
117 0.82553422
118 8.606105213
119 0.303789827
120 7.027671553
121 0.819942567
122 0.571292348
123 2.210028361
124 0.18585718
125 8.386385007
126 8.929061315
127 0.441557009
129 2.340914576

Total number of rows: 8452

Table truncated, full table size 143 Kbytes.




Supplementary file Size Download File type/resource
GSM636751.txt.gz 421.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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