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Sample GSM636745 Query DataSets for GSM636745
Status Public on Dec 09, 2010
Title colon_cornoil_10wk [132]
Sample type RNA
 
Source name colonic mucosa, fed corn oil, terminated 10 wk after carcinogen injection
Organism Rattus norvegicus
Characteristics diet: corn oil
time point: terminated 10 wk after carcinogen injection
strain: Sprague Dawley
gender: Weanling male
tissue: colon
Treatment protocol Rats were treated with the carcinogen azoxymethane at 15 mg/kg body weight and terminated 12 h later or were given a second injection and terminated 9 wk later.
Growth protocol Rats were fed diets that differed in type of fat (corn oil or fish oil or olive oil).
Extracted molecule total RNA
Extraction protocol Ambion Totally RNA kit followed by DNAFree treatment.
Label biotin, Alexa-647 Streptavidin
Label protocol Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Data processing Median normalized values within each array as calculated in Microsoft Excel version 2003
 
Submission date Dec 08, 2010
Last update date Dec 09, 2010
Contact name Jennifer Goldsby
E-mail(s) jsgoldsby@tamu.edu
Phone 979-845-3908
Organization name Texas A&M University
Department Nutrition and Food Sciences
Street address 112 Cater-Mattil
City College Station
State/province TX
ZIP/Postal code 77845
Country USA
 
Platform ID GPL2890
Series (1)
GSE25912 Classification of diet modified gene signatures at the colon cancer initiation and progression stages

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
109 1.500671064
110 2.383907354
111 1.65575833
112 1.475082166
113 3.392481708
114 1.174154041
115 0.883209358
116 1.770625469
117 0.910374121
118 5.621644723
119 0.285563321
120 5.67447549
121 0.773854039
122 0.426528339
123 1.515770012
124 0.121404864
125 6.75118264
126 7.035302036
127 0.288432057
129 1.898699924

Total number of rows: 8452

Table truncated, full table size 143 Kbytes.




Supplementary file Size Download File type/resource
GSM636745.txt.gz 430.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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