|
Status |
Public on Dec 09, 2010 |
Title |
colon_cornoil_10wk [132] |
Sample type |
RNA |
|
|
Source name |
colonic mucosa, fed corn oil, terminated 10 wk after carcinogen injection
|
Organism |
Rattus norvegicus |
Characteristics |
diet: corn oil time point: terminated 10 wk after carcinogen injection strain: Sprague Dawley gender: Weanling male tissue: colon
|
Treatment protocol |
Rats were treated with the carcinogen azoxymethane at 15 mg/kg body weight and terminated 12 h later or were given a second injection and terminated 9 wk later.
|
Growth protocol |
Rats were fed diets that differed in type of fat (corn oil or fish oil or olive oil).
|
Extracted molecule |
total RNA |
Extraction protocol |
Ambion Totally RNA kit followed by DNAFree treatment.
|
Label |
biotin, Alexa-647 Streptavidin
|
Label protocol |
Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
|
|
|
Hybridization protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
Scan protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
|
Data processing |
Median normalized values within each array as calculated in Microsoft Excel version 2003
|
|
|
Submission date |
Dec 08, 2010 |
Last update date |
Dec 09, 2010 |
Contact name |
Jennifer Goldsby |
E-mail(s) |
jsgoldsby@tamu.edu
|
Phone |
979-845-3908
|
Organization name |
Texas A&M University
|
Department |
Nutrition and Food Sciences
|
Street address |
112 Cater-Mattil
|
City |
College Station |
State/province |
TX |
ZIP/Postal code |
77845 |
Country |
USA |
|
|
Platform ID |
GPL2890 |
Series (1) |
GSE25912 |
Classification of diet modified gene signatures at the colon cancer initiation and progression stages |
|