|
| Status |
Public on Dec 09, 2010 |
| Title |
colon_fishoil_12h [201] |
| Sample type |
RNA |
| |
|
| Source name |
colonic mucosa, fed fish oil, terminated 12 h after carcinogen injection
|
| Organism |
Rattus norvegicus |
| Characteristics |
diet: fish oil
time point: terminated 12 h after carcinogen injection
strain: Sprague Dawley
gender: Weanling male
tissue: colon
|
| Treatment protocol |
Rats were treated with the carcinogen azoxymethane at 15 mg/kg body weight and terminated 12 h later or were given a second injection and terminated 9 wk later.
|
| Growth protocol |
Rats were fed diets that differed in type of fat (corn oil or fish oil or olive oil).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion Totally RNA kit followed by DNAFree treatment.
|
| Label |
biotin, Alexa-647 Streptavidin
|
| Label protocol |
Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
|
| |
|
| Hybridization protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
| Scan protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
|
| Data processing |
Median normalized values within each array as calculated in Microsoft Excel version 2003
|
| |
|
| Submission date |
Dec 08, 2010 |
| Last update date |
Dec 09, 2010 |
| Contact name |
Jennifer Goldsby |
| E-mail(s) |
jsgoldsby@tamu.edu
|
| Phone |
979-845-3908
|
| Organization name |
Texas A&M University
|
| Department |
Nutrition and Food Sciences
|
| Street address |
112 Cater-Mattil
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77845 |
| Country |
USA |
| |
|
| Platform ID |
GPL2890 |
| Series (1) |
| GSE25912 |
Classification of diet modified gene signatures at the colon cancer initiation and progression stages |
|
