|
| Status |
Public on Nov 23, 2013 |
| Title |
Phosphate starvation for 10 day rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Phosphate starvation for 10day
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
cultivar: Nipponbare
tissue: seedling
treatment: Phosphate starvation
time: 10 day
|
| Growth protocol |
2 weeks-old rice seedling, growing on the liquid media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
We followed the manufacturer's instructions for the Agilent two-color experiment.
|
| |
|
| Channel 2 |
| Source name |
non-treatment
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
tissue: seedling
cultivar: Nipponbare
treatment: untreated
|
| Growth protocol |
2 weeks-old rice seedling, growing on the liquid media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
We followed the manufacturer's instructions for the Agilent two-color experiment.
|
| |
|
| |
| Hybridization protocol |
We followed the manufacturer's instructions for the Agilent two-color experiment.
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner. Images were quantified using Agilent Feature Extraction Software.
|
| Description |
Biological replicate 2 of 3. Phosphate starvation treatment for 10day/non-treatment
|
| Data processing |
Agilent Feature Extraction Software was used for background subtraction and normalization.
|
| |
|
| Submission date |
Nov 29, 2010 |
| Last update date |
Nov 23, 2013 |
| Contact name |
Youko Oono |
| URL |
http://www.nias.affrc.go.jp/index_e.html
|
| Organization name |
National Institute of Agrobiological Sciences
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6864 |
| Series (1) |
| GSE25647 |
Phosphate starvation/sufficient treatment of rice seedling |
|
