|
| Status |
Public on Jun 11, 2022 |
| Title |
oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h
|
| Organism |
Oryza sativa |
| Characteristics |
sample group: oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h
|
| Treatment protocol |
The leaf disks (6 mmϕ) were excised from the uppermost rice leaf blades of 1 month-old plants. Then the leaf disks were floated on 0.1%(v/v) DMSO or 0.5 mM MeJA
|
| Growth protocol |
Wild-type rice plants (Oryza sativa L., 'Nipponbare') and rice COI mutants were grown in a glass house under natural daylight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Low RNA input Linear Amplification/Labeling Kit (Agilent)
|
| |
|
| Hybridization protocol |
Agilent protocol G4140-90040
|
| Scan protocol |
Agilent Technologies Scanner G2505C US22502560
|
| Description |
Biological replicate 2 of 3.
|
| Data processing |
After data was analyzed by Fearure Extraction software (Agilent), data normalization was performed using Subio Platform with Basic Plug-in (Subio Inc).
|
| |
|
| Submission date |
Jun 09, 2022 |
| Last update date |
Jun 11, 2022 |
| Contact name |
Koji Miyamoto |
| E-mail(s) |
miyamoto@nasu.bio.teikyo-u.ac.jp
|
| Phone |
+81-28-627-7566
|
| Organization name |
Teikyo University
|
| Department |
Department of Biosciences
|
| Street address |
1-1 Toyosatodai
|
| City |
Utsunomiya |
| State/province |
Tochigi |
| ZIP/Postal code |
320-8551 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8852 |
| Series (1) |
| GSE205750 |
Effect of methyl jasmonate treatment on rice leaves of wild-type and rice COI mutatns |
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