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Status |
Public on Jun 11, 2022 |
Title |
oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h replicate 1 |
Sample type |
RNA |
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|
Source name |
oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h
|
Organism |
Oryza sativa |
Characteristics |
sample group: oscoi1a #1 leaves treated with 0.5 mM MeJA for 24 h
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Treatment protocol |
The leaf disks (6 mmϕ) were excised from the uppermost rice leaf blades of 1 month-old plants. Then the leaf disks were floated on 0.1%(v/v) DMSO or 0.5 mM MeJA
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Growth protocol |
Wild-type rice plants (Oryza sativa L., 'Nipponbare') and rice COI mutants were grown in a glass house under natural daylight.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Plant Mini Kit (Qiagen)
|
Label |
Cy3
|
Label protocol |
Low RNA input Linear Amplification/Labeling Kit (Agilent)
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|
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Hybridization protocol |
Agilent protocol G4140-90040
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Scan protocol |
Agilent Technologies Scanner G2505C US22502560
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Description |
Biological replicate 1 of 3.
|
Data processing |
After data was analyzed by Fearure Extraction software (Agilent), data normalization was performed using Subio Platform with Basic Plug-in (Subio Inc).
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Submission date |
Jun 09, 2022 |
Last update date |
Jun 11, 2022 |
Contact name |
Koji Miyamoto |
E-mail(s) |
miyamoto@nasu.bio.teikyo-u.ac.jp
|
Phone |
+81-28-627-7566
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Organization name |
Teikyo University
|
Department |
Department of Biosciences
|
Street address |
1-1 Toyosatodai
|
City |
Utsunomiya |
State/province |
Tochigi |
ZIP/Postal code |
320-8551 |
Country |
Japan |
|
|
Platform ID |
GPL8852 |
Series (1) |
GSE205750 |
Effect of methyl jasmonate treatment on rice leaves of wild-type and rice COI mutatns |
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