|
| Status |
Public on May 17, 2023 |
| Title |
sample23_normal |
| Sample type |
SRA |
| |
|
| Source name |
liver grafts,normal,patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver graft
disease: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver grafts using TRIzol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastp (v0.21.0)
Trimmed sequence reads were mapped to GRCh38/rn6 using STAR(v2.7.7a)
Read count extraction and normalization were performed using RSEM(v1.3.3)
Assembly: GRCh38/rn6
Supplementary files format and content: tab-delimited text files include read count for each sample
|
| |
|
| Submission date |
May 26, 2022 |
| Last update date |
May 17, 2023 |
| Contact name |
Yueqin ZHU |
| Organization name |
Hong Kong University
|
| Street address |
Sassoon Road
|
| City |
Hong Kong |
| State/province |
China |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE204919 |
Deep sequencing for mRNA in steatotic/normal liver grafts from patients and rat transplant model |
|
| Relations |
| BioSample |
SAMN28685769 |
| SRA |
SRX15474014 |
