|
Status |
Public on Dec 26, 2012 |
Title |
NB-#15BTH(24hr)-4 |
Sample type |
RNA |
|
|
Source name |
leaves
|
Organism |
Oryza sativa |
Characteristics |
cultivar: Nipponbare tissue: leaf protocol: control treatment: BTH developmental stage: 4th leaves at 4-leaf stage time: 24 h
|
Treatment protocol |
seedlings at the four-leaf stage were sprayed with 0.5 mM BTH in 0.5% acetone / 0.05% Tween20 (BTH) or the solvent only (mock) for 24 h
|
Growth protocol |
Rice plants were grown on soil from seeds.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using an RNeasy plant mini kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
The RNAs (400-ng aliquots) were labeled with a Low RNA Input Linear Amplification/Labeling Kit (Agilent Technologies)
|
|
|
Hybridization protocol |
Agilent protocol G4140-90040
|
Scan protocol |
Slides were scanned immediately after washing on Agilent Scanner model G2505B and software G2565BA using default parameters
|
Description |
n/a
|
Data processing |
The scanned images were analyzed with Feature Extraction software (version 9.1; Agilent Technologies) using default parameters.
|
|
|
Submission date |
Aug 20, 2010 |
Last update date |
Dec 26, 2012 |
Contact name |
Hiroshi Takatsuji |
E-mail(s) |
takatsuh@affrc.go.jp
|
Organization name |
National Institute of Agrobiological Sciences
|
Department |
Division of Plant Sciences
|
Lab |
Plant Disease Resistance Research Unit
|
Street address |
2-1-2 Kannondai
|
City |
Tsukuba |
State/province |
Ibaraki |
ZIP/Postal code |
305-8602 |
Country |
Japan |
|
|
Platform ID |
GPL10843 |
Series (1) |
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