|
| Status |
Public on Dec 26, 2012 |
| Title |
WRKY45kd#3-BTH(24hr)-1 |
| Sample type |
RNA |
| |
|
| Source name |
leaves
|
| Organism |
Oryza sativa |
| Characteristics |
developmental stage: 4th leaves at 4-leaf stage
cultivar: Nipponbare
tissue: leaf
protocol: WRKY45kd#3 knockdown
treatment: BTH
time: 24 h
|
| Treatment protocol |
seedlings at the four-leaf stage were sprayed with 0.5 mM BTH in 0.5% acetone / 0.05% Tween20 (BTH) or the solvent only (mock) for 24 h
|
| Growth protocol |
Rice plants were grown on soil from seeds.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy plant mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
The RNAs (400-ng aliquots) were labeled with a Low RNA Input Linear Amplification/Labeling Kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Agilent protocol G4140-90040
|
| Scan protocol |
Slides were scanned immediately after washing on Agilent Scanner model G2505B and software G2565BA using default parameters
|
| Description |
n/a
|
| Data processing |
The scanned images were analyzed with Feature Extraction software (version 9.1; Agilent Technologies) using default parameters.
|
| |
|
| Submission date |
Aug 20, 2010 |
| Last update date |
Dec 26, 2012 |
| Contact name |
Hiroshi Takatsuji |
| E-mail(s) |
takatsuh@affrc.go.jp
|
| Organization name |
National Institute of Agrobiological Sciences
|
| Department |
Division of Plant Sciences
|
| Lab |
Plant Disease Resistance Research Unit
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10843 |
| Series (1) |
|
