|
| Status |
Public on Jun 15, 2010 |
| Title |
SIGC rep2 |
| Sample type |
RNA |
| |
|
| Source name |
cultivar : Hermes
|
| Organism |
Linum usitatissimum |
| Characteristics |
cultivar: Hermes
tissue: inner stem tissue
developmental stage: green capsule stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and DNA was removed byDNase digestion with the DNA-Free set (Ambion). RNA quality and concentration was determined by analysis with an Experion bioanalyzer (Biorad) using RNA Std chips.
|
| Label |
Cy3
|
| Label protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| |
|
| Hybridization protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| Scan protocol |
According to standard NimbleGen protocols (www.nimblegen.com)
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.). Processed data then consist of log2(intensities) files (.calls files).
|
| |
|
| Submission date |
May 17, 2010 |
| Last update date |
May 17, 2010 |
| Contact name |
Stephane Fenart |
| E-mail(s) |
stephane.fenart@univ-lille1.fr
|
| Phone |
0033320434028
|
| Organization name |
University Lille 1
|
| Department |
Biology
|
| Lab |
UMR 1281
|
| Street address |
Cité Scientifique
|
| City |
Villeneuve d'Ascq |
| ZIP/Postal code |
59665 |
| Country |
France |
| |
|
| Platform ID |
GPL10419 |
| Series (1) |
| GSE21868 |
gene expression analysis in different tissues of Flax (Linum usitatissimum L.) |
|
