|
| Status |
Public on Apr 27, 2010 |
| Title |
LMP_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LMP_rep2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Cells were maintained in DMEM supplemented with 10% FCS and grown at 37 ºC in a humidified incubator with 5% CO2. At approxiamtely 90% confluence, cells were passaged 1:10.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested by trypsinization and RNA was extracted using Qiagen Rneasy kit following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent protocol
|
| |
|
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Agilent protocol
|
| Description |
LMP_rep2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.5.1.1 (Agilent) using default parameters (protocol GE1_105_Jan09 and Grid: 014850_D_F_20090416) to obtain background corrected and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Apr 05, 2010 |
| Last update date |
Jul 19, 2013 |
| Contact name |
Peter J Park |
| E-mail(s) |
peter_park@harvard.edu
|
| Phone |
617-432-7373
|
| Organization name |
Harvard Medical School
|
| Department |
Center for Biomedical Informatics
|
| Street address |
10 Shattuck St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE21201 |
CpG islands recruit a histone H3 lysine 36 demethylase [Agilent data] |
| GSE21202 |
CpG islands recruit a histone H3 lysine 36 demethylase |
|
