|
| Status |
Public on Apr 30, 2021 |
| Title |
Sample_16k_PRL_2 |
| Sample type |
SRA |
| |
|
| Source name |
NRCMs
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: 16k PRL
cell type: neonate rat cardiomyocytes (NRCMs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from hears by using a miRNeasy micro Kit (Qiagen) with the DNase digestion step, according to the manufacturer’s instructions.
Libraries were prepared according to Illumina's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Gene expression data of NRCMs
|
| Data processing |
Illumina CASAVA1.8.2 software used for basecalling and demultiplexing.
Reads were trimmed with Trimmomatic.
The trimmed reads were aligned to the mouse reference genome mm10 with HISAT2.
Sequencing tracks normalized by the mapped read counts were generated with deepTools.
Genome_build: Genome Reference Consortium Mouse Build 38 (mm10)
Supplementary_files_format_and_content: bigwig
|
| |
|
| Submission date |
Mar 17, 2021 |
| Last update date |
Apr 30, 2021 |
| Contact name |
Takashi Shimizu |
| E-mail(s) |
qinshyui@gmail.com
|
| Phone |
+80358412881
|
| Organization name |
University of Tokyo
|
| Street address |
2-11-16 Yayoi
|
| City |
Bunkyo-ku |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE169063 |
Comparison of gene expression profiles of neonate rat cardiomyocytes (NRCMs) transfected with LacZ, 23k prolactin (PRL), or 16k PRL. |
|
| Relations |
| BioSample |
SAMN18326697 |
| SRA |
SRX10364435 |
