|
| Status |
Public on May 03, 2021 |
| Title |
PLASMA-HD-MN-20-06-13 |
| Sample type |
RNA |
| |
|
| Source name |
PLASMA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PLASMA
disease state: healthy
|
| Treatment protocol |
ex-vivo
|
| Growth protocol |
Blood samples were obtained by venous punctures in EDTA-tubes for plasma preparation. Cell- and platelet-free plasma was prepared following a step centrifugation protocol: samples were centrifuged at 1.500 g for 15′ at 4 °C and supernatant will be stored at −80 °C. RNA was extracted using Plasma/Serum Circulating RNA purification Kit (NORGEN) following the manufacturer’s instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell- and platelet-free plasma was prepared following a step centrifugation protocol: samples were centrifuged at 1.500 g for 15′ at 4 °C and supernatant will be stored at −80 °C. RNA was extracted using Plasma/Serum Circulating RNA purification Kit (NORGEN) following the manufacturer’s instructions. RNA quality and purity were assessed with the use of the RNA 6000 Nano assay on Agilent 2100 Bioanalyzer (Agilent).
|
| Label |
Biotin
|
| Label protocol |
Labeled RNA from each sample was analyzed by GeneChipmiRNA 2.0 Array (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
The arrays were stained in the Fluidics Station 450 and then scanned on the GeneChip® Scanner 3000 (Affymetrix, USA)
|
| Scan protocol |
The arrays were stained in the Fluidics Station 450 and then scanned on the GeneChip® Scanner 3000 (Affymetrix, USA)
|
| Description |
miRNAs expression analysis
|
| Data processing |
MicroRNA probe outliers were defined from the manufacturer's instructions (Affymetrix), and further analysis included data summarization, normalization and quality control using the web-based miRNA QC Tool software (Affymetrix).
|
| |
|
| Submission date |
Feb 26, 2021 |
| Last update date |
May 04, 2021 |
| Contact name |
Silvia Romano |
| E-mail(s) |
silvia.romano@uniroma1.it
|
| Organization name |
University of Rome La Sapienza
|
| Department |
NESMOS
|
| Street address |
Via di Grottarossa 1035
|
| City |
Rome |
| ZIP/Postal code |
00189 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE167630 |
Circulating non-coding RNAs in Huntington disease |
|
