|
Status |
Public on Mar 08, 2010 |
Title |
NUID-0000-0094-1625 |
Sample type |
RNA |
|
|
Source name |
45 % Liver / 45 % Brain / 10 % Lung
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: 45 % Liver / 45 % Brain / 10 % Lung
|
Treatment protocol |
Untreated
|
Growth protocol |
9-12 week old animals
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction using Trizol extraction followed by RNEasy cleanup
|
Label |
biotin
|
Label protocol |
cDNA synthesis and labeling was done with a starting amount of 1 μg, using the Affymetrix labeling kit, following the manufacturer’s instructions.
|
|
|
Hybridization protocol |
Labeled cRNA was hybridized to rat specific RAE230 whole genome expression arrays (Affymetrix).
|
Scan protocol |
GeneChips were scanned
|
Description |
Mixture
|
Data processing |
The data was analyzed using RMA using R Bioconductor
|
|
|
Submission date |
Jan 11, 2010 |
Last update date |
Mar 08, 2010 |
Contact name |
Shai Shen-Orr |
Organization name |
Stanford University
|
Street address |
251 Campus Dr. Rm.X-251
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE19830 |
Expression data from pure/mixed brain, liver and lung to test feasability and sensitivity of statistical deconvolution |
|