|
| Status |
Public on Mar 08, 2010 |
| Title |
NUID-0000-0094-1614 |
| Sample type |
RNA |
| |
|
| Source name |
70 % Liver / 5 % Brain / 25 % Lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: 70 % Liver / 5 % Brain / 25 % Lung
|
| Treatment protocol |
Untreated
|
| Growth protocol |
9-12 week old animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using Trizol extraction followed by RNEasy cleanup
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and labeling was done with a starting amount of 1 μg, using the Affymetrix labeling kit, following the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized to rat specific RAE230 whole genome expression arrays (Affymetrix).
|
| Scan protocol |
GeneChips were scanned
|
| Description |
Mixture
|
| Data processing |
The data was analyzed using RMA using R Bioconductor
|
| |
|
| Submission date |
Jan 11, 2010 |
| Last update date |
Mar 08, 2010 |
| Contact name |
Shai Shen-Orr |
| Organization name |
Stanford University
|
| Street address |
251 Campus Dr. Rm.X-251
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE19830 |
Expression data from pure/mixed brain, liver and lung to test feasability and sensitivity of statistical deconvolution |
|
