|
| Status |
Public on Jan 31, 2010 |
| Title |
SKO-transduced MEFs, Vc+, D10 |
| Sample type |
RNA |
| |
|
| Source name |
SKO-transduced MEFs, Vc treated, lysed on D10
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MEF
transduction: SKO (sox2, klf4 and oct4)
treatment: vitamin C
time: Day 10
|
| Treatment protocol |
Vc was added to mESC medium at a final concentration of 50 μg/ml. At D2 after the 2nd infection, transduced MEFs were changed to mESC medium with or without Vc. Vc was continuously added in the Vc treatment group.
|
| Growth protocol |
MEFs were cultured in DMEM+10% FBS+NEAA+GlutaMax+penicillin/streptomycin (P/S); after two-rounds of infections, GFP- or SKO-transduced MEFs were cultured in mESC standard medium (DMEM+15% FBS+NEAA+GlutaMax+Sodium pyruvate+P/S+β-mercaptoethanol+1,000U/ml LIF) with or without Vc.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Whole Transcript (WT) Sense Target Labeling Assay Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip MoGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
Gene expression data from SKO-transduced MEFs, Vc+, D10.
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Partek software.
|
| |
|
| Submission date |
Dec 08, 2009 |
| Last update date |
Dec 09, 2009 |
| Contact name |
Duanqing Pei |
| E-mail(s) |
duanqing.pei@gmail.com
|
| Phone |
086-20-32290520
|
| Fax |
086-20-32290606
|
| Organization name |
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
|
| Department |
Center for Molecular Medicine
|
| Lab |
Lingwen Zeng's Lab
|
| Street address |
Room 301, International Business Incubator, Guangzhou science park
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510663 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE19377 |
DNA microarrays time course of SKO-infected MEFs treated with Vc |
| GSE19379 |
DNA microarrays time course of SKO-infected MEFs and MaF pre-iPSCs treated with Vc |
|
