|
| Status |
Public on Dec 31, 2009 |
| Title |
intestine_age-1_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
young adult hermaphrodite
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: intestine-restricted age-1 expression
|
| Growth protocol |
Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
|
| Label |
Cy3
|
| Label protocol |
cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
|
| |
|
| Channel 2 |
| Source name |
young adult hermaphrodite
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: wildtype (N2)
|
| Growth protocol |
Eggs obtained by bleach prep of gravid hermaphrodites and hatched overnight in S basal to synchronize at L1. L1's were plated onto NG agar with OP50 food and grown 72 hours to L4/young adult stage at 20˚C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Absolutely RNA miniprep kit (Stratagene, La Jolla, CA)
|
| Label |
Cy5
|
| Label protocol |
cDNA was labeled with the Quick Amp 2 Color Labeling Kit (Agilent Technologies, Santa Clara, CA)
|
| |
|
| |
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
standard Agilent protocol
|
| Description |
n/a
|
| Data processing |
Probes with raw fluorescence signals above 100 in wildtype were selected for further analysis as being reliable signals. Expression ratios for each gene were calculated in all 3 strains with respect to wildtype controls. Expression ratios were calculated as z-scores for a statistical evaluation of relative expression and as fold-changes for standardized displays of relative expression. To determine fold-change, raw fluorescence values were first normalized by dividing the fluorescence intensity for a given sample by the average intensity of all samples for the array. Fold-change ratios were then determined by dividing mean normalized value for all replicates of test versus control conditions.
|
| |
|
| Submission date |
Sep 21, 2009 |
| Last update date |
Sep 21, 2009 |
| Contact name |
Catherine Wolkow |
| E-mail(s) |
wolkowca@mail.nih.gov
|
| Organization name |
NIA IRP, NIH
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL2875 |
| Series (1) |
| GSE18200 |
Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity |
|
