|
| Status |
Public on Aug 19, 2009 |
| Title |
04-174 |
| Sample type |
RNA |
| |
|
| Source name |
t(9;22)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
ethnicity: Chinese
disease: pediatric acute lymphoblastic leukemia
chinese pediatric all subtype: t(9;22)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cryopreserved mononuclear cell suspensions from BM samples using Trizol (Invitrogen) and purified with the RNeasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were synthesized with One-Cycle Target Labeling and Control Reagents (Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled RNA was then fragmented and hybridized to HG-U133A 2.0 oligonucleotide arrays (Affymetrix Incorporated, Santa Clara, CA) according to Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
Chinese ALL sample.
Sample ID: 04-174
|
| Data processing |
Data were processed by using the robust multi-array analysis (RMA) from the Bioconductor package.
|
| |
|
| Submission date |
Aug 18, 2009 |
| Last update date |
Aug 25, 2009 |
| Contact name |
Nan Qiao |
| Organization name |
Institute of Genetics and Developmental Biology
|
| Department |
System Biology
|
| Street address |
Datun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE17703 |
Bone marrow gene expression of pediatric acute lymphoblastic leukemia (ALL) |
|
