|
Status |
Public on Mar 14, 2020 |
Title |
34T: 22 month old tricep muscle rep4 |
Sample type |
SRA |
|
|
Source name |
tricep muscle
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Wistar gender: male tissue: tricep muscle age (month): 22 animal id: 34
|
Extracted molecule |
polyA RNA |
Extraction protocol |
mRNA was extracted using the miRNeasy kit from Qiagen, following the manufacturer's instructions. TruSeq RNA Library Preparation Kit v2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Illumina Casava 1.8 software used for basecalling and demultiplexing. Sequenced reads were trimmed for adaptor sequence, and mapped to Rnor 6.0 whole genome using RNA-STAR v2.3.0e with parameters --genomeLoad NoSharedMemory --runThreadN 12 Counts within genes were generated using HTSeq, mode union, strand no, annotation file Rattus_norvegicus.Rnor_6.0.80.gtf Genome_build: RGSC 6.0 Supplementary_files_format_and_content: tab-delimited text file including counts per gene for each sample quantified by HTSeq
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|
|
Submission date |
Mar 13, 2020 |
Last update date |
Mar 14, 2020 |
Contact name |
Jerome Feige |
Organization name |
Nestle Institute Of Health Sciences
|
Department |
Musculo-Skeletal Health
|
Street address |
EPFL Innovation Park, Building H
|
City |
Lausanne |
ZIP/Postal code |
1015 |
Country |
Switzerland |
|
|
Platform ID |
GPL18694 |
Series (2) |
GSE146976 |
Comparison of age-related changes in gene expression of 2 rat skeletal muscles (20 months, 22 months) |
GSE146977 |
Comparison of age-related changes in gene expression of 2 rat skeletal muscles |
|
Relations |
BioSample |
SAMN14376410 |
SRA |
SRX7911196 |