|
| Status |
Public on Feb 12, 2020 |
| Title |
20_02_2013_D10_S5.CEL SSc |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
disease state: SSc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a PAXGene Blood RNA kit (PreAnalytiX GmbH) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Gene expression analysis was performed using the Thermo Fischer Scientific Human Genome U219 96-array plate. To process the RNA samples, we used the Beckman Biomek FX TPE System (Beckman Coulter Inc., Indianapolis) by the manufacturer HT-IVT express 2.0.6 standard protocol from Thermo Fischer Scientific.
|
| |
|
| Hybridization protocol |
RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
|
| Scan protocol |
RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
|
| Description |
Gene expression data of SSc patients compared to healthy controls
|
| Data processing |
All statistical analyses were carried out using the R system for statistical computing, version 3.2.3, whereas support vector machines (SVM) were trained and evaluated using the R package 'e1071', version 1.6-8. We used Ingenuity pathway analysis (IPA) to collect more information about signaling pathways, networks and gene functions
|
| |
|
| Submission date |
Feb 11, 2020 |
| Last update date |
Feb 12, 2020 |
| Contact name |
Pia Moinzadeh |
| E-mail(s) |
pia.moinzadeh@uk-koeln.de
|
| Organization name |
University of Cologne
|
| Street address |
Kerpener Str 62
|
| City |
Cologne |
| ZIP/Postal code |
50937 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE145120 |
Gene expression data of different SSc subsets |
|
