|
| Status |
Public on Apr 02, 2010 |
| Title |
PPMS, P047 |
| Sample type |
RNA |
| |
|
| Source name |
blood, PPMS
|
| Organism |
Homo sapiens |
| Characteristics |
ms subtype: PPMS
tissue: whole blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the whole blood in PAXgene tubes. Total RNA quality was assessed and its concentration was measured using the Agilent RNA 6000 series II Nano kit (Agilent Technologies, CA). 250 ng total RNA from each sample was biotinylated and amplified using Illumina® TotalPrep RNA Amplification Kit (Ambion, TX). Total RNA was reverse transcribed to synthesize the first strand of cDNA, followed by a second strand synthesis.
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was then transcribed and amplified in vitro to synthesize biotin-labelled complementary mRNA (cRNA).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
P047
|
| Data processing |
The data were normalised using cubic spline normalisation with Illumina beadstudio.
Non-normalized data is available in the supplementary file 'GSE17048_non-normalized_data.txt', which is linked to the Series GSE17048 record.
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Apr 02, 2010 |
| Contact name |
David Richmond Booth |
| E-mail(s) |
david_booth@wmi.usyd.edu.au
|
| Phone |
0061 2 98458498
|
| Fax |
0061 2 98913889
|
| Organization name |
Westmead Millennium Institute
|
| Department |
Immunology
|
| Street address |
Darcy Rd
|
| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE17048 |
Multiple Sclerosis Blood Cell mRNA Transcriptome |
|
