|
| Status |
Public on Jun 24, 2010 |
| Title |
chitin treatment (vector control) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
water treatment rice cells
|
| Organism |
Oryza sativa |
| Characteristics |
cell type: vector control
|
| Treatment protocol |
treated with water for 2hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Fluorescence labeled according to the Agilent manufacture’s protocol.
|
| |
|
| Channel 2 |
| Source name |
chitin treatment rice cells
|
| Organism |
Oryza sativa |
| Characteristics |
cell type: vector control
|
| Treatment protocol |
treated with chitin for 2hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
Fluorescence labeled according to the Agilent manufacture’s protocol.
|
| |
|
| |
| Hybridization protocol |
The microarrays were hybridized according to the Agilent manufacture’s protocol.
|
| Scan protocol |
The slides were scanned by an Agilent scanner.
|
| Description |
Rice suspension cultured cells (Oryza sativa L. cv. Nipponnbare) used for total RNA extraction were maintained using a modified N-6 medium and incubated on a rotary shaker at 25 C and 120 rpm in a dark. Cells were transferred to a fresh medium every week. Those cells 4 or 5 days after transfer to the new medium were used experiment.40mg of cultured rice cells were transferred to 1 ml of the fresh medium in a 2 ml centrifuge tube and pre-incubated for 30 min at 25 C on a thermomixer shaken at 750 rpm. N-acetylchitooctaose elicitor (100nM) was added to the culture medium and incubated for 2hr.Total RNA was extracted from rice cells by RNeasy Plant Mini Kit (QIAGEN Inc., Valencia, CA, USA).
|
| Data processing |
Data was analyzed by Fearure Extraction software
|
| |
|
| Submission date |
Jun 24, 2009 |
| Last update date |
Feb 25, 2011 |
| Contact name |
Hanae Kaku |
| E-mail(s) |
kaku@isc.meiji.ac.jp
|
| Phone |
+81-44-934-7805
|
| Fax |
+81-44-934-7805
|
| Organization name |
Meiji University
|
| Department |
Life Sciences
|
| Street address |
1-1-1, Higashi-Mita, Tama-ku
|
| City |
Kawasaki |
| State/province |
Kanagawa |
| ZIP/Postal code |
214-8571 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6864 |
| Series (1) |
| GSE16800 |
The function of OsCERK1 in the chitin elicitor signaling in Rice |
|
