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Sample GSM4137576 Query DataSets for GSM4137576
Status Public on Jul 20, 2020
Title GSS1403_128_F_AE
Sample type RNA
 
Source name LCM-separated epidermis from human arm
Organism Homo sapiens
Characteristics tissue: LCM-separated epidermis
collection time: NA
gender: female
Treatment protocol Punch biopsy samples were frozen in OCT media. Laser capture microdissection (LCM) was performed to isolate the epidermal compartments for gene expression profiling.
Extracted molecule total RNA
Extraction protocol Total RNA from LCM samples was isolated utilizing the Pico Pure RNA Isolation Kit (Life Technologies, Grand Island, NY) according to manufacturer's recommendations
Label biotin
Label protocol Biotinylated cRNA were prepared using the Affymetrix HT 3’ IVT Express kit (Cat. #901253) on a Beckman Biomek FXp Laboratory Automation Workstation (Beckman Cat. #A31842).
 
Hybridization protocol Biotinylated cRNA was fragmented by limited alkaline hydrolysis and then hybridized overnight to Affymetrix GeneTitan U219 microarray plates using the Affymetrix GeneTitan instrument
Scan protocol ArrayPlates were scanned using the Affymetrix GeneTitan Instrument utilizing AGCC GeneTitan Instrument Control software version 4.2.0.1566
Description batch_subjectID_gender_sampling site
Data processing The RMA algorithm was used as the processing protocol to normalize the data for subsequent analysis.
 
Submission date Oct 23, 2019
Last update date Jul 21, 2020
Contact name Joe Sherrill
E-mail(s) sherrill.jd@pg.com
Phone 5136222038
Organization name Procter and Gamble
Street address 8700 Mason-Montgomery Rd
City Mason
State/province Ohio
ZIP/Postal code 45040
Country USA
 
Platform ID GPL13667
Series (2)
GSE139301 The effect of circadian rhythm on gene expression in human skin III
GSE139305 The effect of circadian rhythm on gene expression in human skin.

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
11715100_at 2.269279891
11715101_s_at 2.412181143
11715102_x_at 2.250341864
11715103_x_at 2.652145703
11715104_s_at 2.066660507
11715105_at 2.184418811
11715106_x_at 2.952969326
11715107_s_at 2.272011094
11715108_x_at 2.010450874
11715109_at 2.415983983
11715110_at 1.846689258
11715111_s_at 2.996373821
11715112_at 2.203703528
11715113_x_at 3.803290857
11715114_x_at 3.72407634
11715115_s_at 1.72478497
11715116_s_at 2.169219943
11715117_x_at 2.268521966
11715118_s_at 2.65488505
11715119_s_at 4.953725903

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM4137576_GSS1403_58.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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