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Sample GSM4137554 Query DataSets for GSM4137554
Status Public on Jul 20, 2020
Title GSS1403_179_F_AE
Sample type RNA
 
Source name LCM-separated epidermis from human arm
Organism Homo sapiens
Characteristics tissue: LCM-separated epidermis
collection time: NA
gender: female
Treatment protocol Punch biopsy samples were frozen in OCT media. Laser capture microdissection (LCM) was performed to isolate the epidermal compartments for gene expression profiling.
Extracted molecule total RNA
Extraction protocol Total RNA from LCM samples was isolated utilizing the Pico Pure RNA Isolation Kit (Life Technologies, Grand Island, NY) according to manufacturer's recommendations
Label biotin
Label protocol Biotinylated cRNA were prepared using the Affymetrix HT 3’ IVT Express kit (Cat. #901253) on a Beckman Biomek FXp Laboratory Automation Workstation (Beckman Cat. #A31842).
 
Hybridization protocol Biotinylated cRNA was fragmented by limited alkaline hydrolysis and then hybridized overnight to Affymetrix GeneTitan U219 microarray plates using the Affymetrix GeneTitan instrument
Scan protocol ArrayPlates were scanned using the Affymetrix GeneTitan Instrument utilizing AGCC GeneTitan Instrument Control software version 4.2.0.1566
Description batch_subjectID_gender_sampling site
Data processing The RMA algorithm was used as the processing protocol to normalize the data for subsequent analysis.
 
Submission date Oct 23, 2019
Last update date Jul 21, 2020
Contact name Joe Sherrill
E-mail(s) sherrill.jd@pg.com
Phone 5136222038
Organization name Procter and Gamble
Street address 8700 Mason-Montgomery Rd
City Mason
State/province Ohio
ZIP/Postal code 45040
Country USA
 
Platform ID GPL13667
Series (2)
GSE139301 The effect of circadian rhythm on gene expression in human skin III
GSE139305 The effect of circadian rhythm on gene expression in human skin.

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
11715100_at 1.945485555
11715101_s_at 2.660774664
11715102_x_at 2.170393138
11715103_x_at 3.137049313
11715104_s_at 2.023662848
11715105_at 2.470338865
11715106_x_at 3.093096227
11715107_s_at 2.406961208
11715108_x_at 2.294566008
11715109_at 2.217183044
11715110_at 2.166286428
11715111_s_at 3.121878024
11715112_at 2.417186331
11715113_x_at 3.991978099
11715114_x_at 3.895595989
11715115_s_at 1.752253439
11715116_s_at 2.130393463
11715117_x_at 1.720039127
11715118_s_at 2.596114459
11715119_s_at 5.591156165

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM4137554_GSS1403_35.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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