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Status |
Public on Jul 20, 2020 |
Title |
GSS1376_195_F_BE |
Sample type |
RNA |
|
|
Source name |
LCM-separated epidermis from human buttock
|
Organism |
Homo sapiens |
Characteristics |
tissue: LCM-separated epidermis collection time: NA gender: female
|
Treatment protocol |
Punch biopsy samples were frozen in OCT media. Laser capture microdissection (LCM) was performed to isolate the epidermal compartments for gene expression profiling.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from LCM samples was isolated utilizing the Pico Pure RNA Isolation Kit (Life Technologies, Grand Island, NY) according to manufacturer's recommendations
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using the Affymetrix HT 3’ IVT Express kit (Cat. #901253) on a Beckman Biomek FXp Laboratory Automation Workstation (Beckman Cat. #A31842).
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|
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Hybridization protocol |
Biotinylated cRNA was fragmented by limited alkaline hydrolysis and then hybridized overnight to Affymetrix GeneTitan U219 microarray plates using the Affymetrix GeneTitan instrument
|
Scan protocol |
ArrayPlates were scanned using the Affymetrix GeneTitan Instrument utilizing AGCC GeneTitan Instrument Control software version 4.2.0.1566
|
Description |
batch_subjectID_gender_sampling site
|
Data processing |
The RMA algorithm was used as the processing protocol to normalize the data for subsequent analysis.
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Submission date |
Oct 23, 2019 |
Last update date |
Jul 21, 2020 |
Contact name |
Joe Sherrill |
E-mail(s) |
sherrill.jd@pg.com
|
Phone |
5136222038
|
Organization name |
Procter and Gamble
|
Street address |
8700 Mason-Montgomery Rd
|
City |
Mason |
State/province |
Ohio |
ZIP/Postal code |
45040 |
Country |
USA |
|
|
Platform ID |
GPL13667 |
Series (2) |
GSE139301 |
The effect of circadian rhythm on gene expression in human skin III |
GSE139305 |
The effect of circadian rhythm on gene expression in human skin. |
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