|
| Status |
Public on Oct 03, 2019 |
| Title |
Aged_A4 |
| Sample type |
SRA |
| |
|
| Source name |
plasma
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: plasma
age: aged (22-months)
vascular origin: artery
strain: Sprague-Dawley
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plasma was isolated from EDTA-anticoagulant blood, and RNA was harvested using TRIzol reagent
RNA libraries were prepared for sequencing using TruseqTM Small RNA sample prep Kit
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
miRNA
|
| Data processing |
Base callings performed using Illumina softwares
Sequenced reads were trimmed for 3' adaptor sequence and masked low-quality sequence using Fastx-Toolkit to gain clean small RNA reads
Clean reads performed identical sequences merge, then compared with Rfam and miRBase to identify miRNAs
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: The xls files summary the counts and TPM value of each miRNA in all 20 samples
|
| |
|
| Submission date |
Oct 02, 2019 |
| Last update date |
Oct 03, 2019 |
| Contact name |
Hao Wang |
| Organization name |
Peking University Third Hosptial
|
| Street address |
No.49 Huayuan North Road
|
| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE138317 |
Transcriptome analysis of common and diverged circulating miRNAs between arterial and venous during aging |
|
| Relations |
| BioSample |
SAMN12896872 |
| SRA |
SRX6937116 |
