|
| Status |
Public on Oct 04, 2005 |
| Title |
09.Koth_59_HS.CEL |
| Sample type |
RNA |
| |
|
| Source name |
isolated alveolar macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
status: Smoker
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from mouse and human alveolar macrophages and DNase-treated using the RNeasy RNA extraction kit (Qiagen). RNA concentration and quality were assessed using the Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
|
| Label protocol |
Labeling was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard labeling protocol for this chip type (www.affymetrix.com)
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChips. RNA was ampli?ed by performing two rounds of in vitro transcription (Ambion Message Amp aRNA kit no. 1750).
|
| Scan protocol |
Scanning was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard scanning protocol for this chip type (www.affymetrix.com)
|
| Description |
Total RNA from freshly isolated macrophages
|
| Data processing |
Bioconductor was used for quality control (affyPLM) and preprocessing (RMA).
|
| |
|
| Submission date |
Jan 04, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael E. Salazar |
| Phone |
(415) 514-4371
|
| URL |
http://arrays.ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Department |
|
| Lab |
Functional Genomics Core Laboratories
|
| Street address |
1550 Fourth Street, RM 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE2125 |
isolated alveolar macrophages |
|
| Relations |
| Reanalyzed by |
GSE46903 |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
