|
| Status |
Public on Nov 04, 2009 |
| Title |
MLN, PCV2 pig, 1 dpi, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
mediastinal lymph node of a pig inoculated with PCV2 at day 1
|
| Organism |
Sus scrofa |
| Characteristics |
cell type: mediastinal lymph node
agent: Inoculated with PCV2
age: 24 seven-day-old
|
| Treatment protocol |
24 seven-day-old, CDCD piglets were used; a first group of pigs (n=8) was kept as un-inoculated controls and the rest of pigs (n=16) were oronasally inoculated with 105.2 TCID50 of the Burgos isolate of PCV2.
|
| Growth protocol |
Mediastinal lymph nodes were collected at necropsy, immediately frozen in liquid nitrogen and kept at -80ºC until usage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RiboPure kit (Ambion Cat #1924) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (One-Cycle Target Labeling kit, Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Chips were scanned using the Genechip Scanner 3000
|
| Description |
Gene expression data of mediastinal lymph node of a pig inoculated with PCV2 at day 1
|
| Data processing |
The data were analyzed with the Data Mining Tool (DMT) from Affymetrix. RMA was used as normalization method.
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Nov 04, 2009 |
| Contact name |
Anna Tomas |
| E-mail(s) |
anna.tomas@cresa.uab.cat
|
| Phone |
+34935814567
|
| Fax |
+34935814490
|
| Organization name |
CReSA
|
| Department |
Viral infections
|
| Street address |
campus UAB
|
| City |
Bellaterra |
| State/province |
Barcelona |
| ZIP/Postal code |
08329 |
| Country |
Spain |
| |
|
| Platform ID |
GPL3533 |
| Series (2) |
| GSE14758 |
Expression data from mediastinal lymph nodes of piglets experimentally infected with porcine circovirus type 2 (PCV2) |
| GSE14791 |
Expression data from mediastinal lymph nodes and blood samples of piglets experimentally infected with PCV2 |
|
