|
| Status |
Public on Jun 22, 2020 |
| Title |
Biopsy_2hrs.reperfusion_patient_15 |
| Sample type |
RNA |
| |
|
| Source name |
Biopsy_2hrs.reperfusion
|
| Organism |
Homo sapiens |
| Characteristics |
paired samples: patient_15
timepoint: 2HR_Reperf
tissue: peripheral lung biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from snap frozen samples using the RNeasy Mini Kit (Qiagen; Hilden, Germany). RNA quality was assessed using Nanodrop spectrophotometer (VWR; Radnor, PA) and Bioanalyzer (Agilent; Santa Clara, CA)
|
| Label |
biotin
|
| Label protocol |
According to manufacturer guidelines
|
| |
|
| Hybridization protocol |
According to manufacturer guidelines
|
| Scan protocol |
According to manufacturer guidelines
|
| Description |
639.rep2h
|
| Data processing |
Affymetrix CEL files were imported and processed using RMA in R version 3.5.1 with the affy package. We used an alternative CDF probset description file from Brainarray (version 20) at the University of Michigan (Nucleic Acid Research 33 (20), e175; http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp): hgu133plus2hsentrezgcdf_20.0.0.zip
|
| |
|
| Submission date |
Feb 25, 2019 |
| Last update date |
Jun 24, 2020 |
| Contact name |
Ricardo Zamel |
| Organization name |
University Health Network
|
| Street address |
101 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL22321 |
| Series (2) |
| GSE127003 |
Expression of Human Lung Before and After Transplantation |
| GSE127243 |
Potential Therapeutic Targets for Donor Lung Repair During Human Ex Vivo Lung Perfusion Prior to Transplantation |
|
