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Status |
Public on Feb 01, 2009 |
Title |
1663_MMF |
Sample type |
RNA |
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Source name |
Carotid Endarterectomy, MMF-treated
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Organism |
Homo sapiens |
Characteristics |
Tissue: human carotid endarterectomy from patient with a carotid artertery stenosis (>70% diameter stenosis on angiography) as defined by the North American Symptomatic Carotid Endarterectomy Trial (NACET).
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Biomaterial provider |
Department of Vascular Surgery at the Sint Antonius Hospital, Nieuwegein, the Netherlands
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Treatment protocol |
The patient was randomly assigned to treatment with 1000 mg mycophenolate mofetil (MMF), 2 weeks before carotid endarterectomy surgery.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA molecules were isolated from N=5 consecutive 6 micron thick frozen cross-sections of the endarterectomy sample by using Trizol reagent (Invitrogen), followed by column purification with DNase-I treatment, RNeasy Micro kit (Qiagen).
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Label |
Biotin
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Label protocol |
The Illumina® TotalPrep™ RNA Amplification Kit (Ambion) was used for the linear amplification of mRNA molecules into antisense biotinylated cRNAs.
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|
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Hybridization protocol |
750ng of biotinylated cRNA was hybridized onto the HumanRef-8 Expression BeadChips according to the Manufacturer.
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Scan protocol |
According to the Manufacturer, performed by ServiceXS http://www.servicexs.com).
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Description |
All hybridizations were performed according to the manufacturer's specifications.
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Data processing |
Gene expression analysis was performed by using Illumina's Beadstudio v3 software, followed by a quantile inter-array normalization (R, Bioconductor).
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Submission date |
Dec 12, 2008 |
Last update date |
Jan 06, 2009 |
Contact name |
Oscar Leonard Volger |
E-mail(s) |
Stephan.schirmer@uks.eu, a.m.vanderLaan@amc.uva.nl
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Organization name |
Academic Medical Center
|
Department |
Cardiology
|
Street address |
Meibergdreef 15
|
City |
Amsterdam |
State/province |
Noor Holland |
ZIP/Postal code |
1105 AZ |
Country |
Netherlands |
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|
Platform ID |
GPL6255 |
Series (1) |
GSE13922 |
The effects of Mycophenolate treatment on the transcriptomes of patients with symptomatic carotid artery stenosis |
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