|
| Status |
Public on Feb 01, 2009 |
| Title |
1658_MMF |
| Sample type |
RNA |
| |
|
| Source name |
Carotid Endarterectomy, MMF-treated
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: human carotid endarterectomy from patient with a carotid artertery stenosis (>70% diameter stenosis on angiography) as defined by the North American Symptomatic Carotid Endarterectomy Trial (NACET).
|
| Biomaterial provider |
Department of Vascular Surgery at the Sint Antonius Hospital, Nieuwegein, the Netherlands
|
| Treatment protocol |
The patient was randomly assigned to treatment with 1000 mg mycophenolate mofetil (MMF), 2 weeks before carotid endarterectomy surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA molecules were isolated from N=5 consecutive 6 micron thick frozen cross-sections of the endarterectomy sample by using Trizol reagent (Invitrogen), followed by column purification with DNase-I treatment, RNeasy Micro kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
The Illumina® TotalPrep™ RNA Amplification Kit (Ambion) was used for the linear amplification of mRNA molecules into antisense biotinylated cRNAs.
|
| |
|
| Hybridization protocol |
750ng of biotinylated cRNA was hybridized onto the HumanRef-8 Expression BeadChips according to the Manufacturer.
|
| Scan protocol |
According to the Manufacturer, performed by ServiceXS http://www.servicexs.com).
|
| Description |
All hybridizations were performed according to the manufacturer's specifications.
|
| Data processing |
Gene expression analysis was performed by using Illumina's Beadstudio v3 software, followed by a quantile inter-array normalization (R, Bioconductor).
|
| |
|
| Submission date |
Dec 12, 2008 |
| Last update date |
Jan 06, 2009 |
| Contact name |
Oscar Leonard Volger |
| E-mail(s) |
Stephan.schirmer@uks.eu, a.m.vanderLaan@amc.uva.nl
|
| Organization name |
Academic Medical Center
|
| Department |
Cardiology
|
| Street address |
Meibergdreef 15
|
| City |
Amsterdam |
| State/province |
Noor Holland |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6255 |
| Series (1) |
| GSE13922 |
The effects of Mycophenolate treatment on the transcriptomes of patients with symptomatic carotid artery stenosis |
|
