|
| Status |
Public on Jan 01, 2009 |
| Title |
SHR - 4 week old - biol rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
isolated glomeruli
|
| Organism |
Rattus norvegicus |
| Characteristics |
non-proteinuric rat strain
|
| Treatment protocol |
The animals were treated as described in Koop, K et al. Selective loss of podoplanin protein expression accompanies proteinuria and precedes alterations in podocyte morphology in a spontaneous proteinuric rat model. Am J Pathol. 2008 Aug;173(2):315-26
|
| Growth protocol |
The animals were treated as described in Koop, K et al. Selective loss of podoplanin protein expression accompanies proteinuria and precedes alterations in podocyte morphology in a spontaneous proteinuric rat model. Am J Pathol. 2008 Aug;173(2):315-26
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the glomeruli using the TRIzol (Invitrogen) method, and purified with Qiagen RNeasy mini columns (Qiagen, www.qiagen.com).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was produced using the Ambion MessageAmp II-Biotin Enhanced kit (Ambion, www.ambion.com), according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization and scanning were performed by the Leiden Genome Technology Center according to standard protocols (www.lgtc.com)
|
| Scan protocol |
Hybridization and scanning were performed by the Leiden Genome Technology Center according to standard protocols (www.lgtc.com)
|
| Description |
SHR - 4 week old - biol rep 2
|
| Data processing |
Preprocessing of the data was performed with the GC Robust Multiarray Average (GCRMA) package in the R-environment (www.r-project.org ; www.bioconductor.org)
|
| |
|
| Submission date |
Dec 03, 2008 |
| Last update date |
Dec 03, 2008 |
| Contact name |
klaas koop |
| Organization name |
Leiden University Medical Center
|
| Department |
Pathology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE13810 |
Microarray analysis of the development of proteinuria in the Dahl salt-sensitive rat |
|
