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Status |
Public on Mar 04, 2019 |
Title |
E16R002b02: B2 HUVEC-2 Static Rcab |
Sample type |
RNA |
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Source name |
Human umbilical vein endothelial cells, RCαβ treated, static condition
|
Organism |
Homo sapiens |
Characteristics |
tissue: Human umbilical vein endothelial cells passage: passage 1
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Treatment protocol |
Endothelial cells stimulated with 200 nM RCαβ or 200 ng/ml HGF
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Growth protocol |
Human umbilical vein endothelial cells (HUVECs) were cultured according to the manufacturer’s instructions (Promocell, passages < 5), in complete endothelial cell growth medium containing 2% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S)
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were collected and total RNA was extracted from the cells.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard GeneChip WT Kit protocol from 100ng total RNA.
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Hybridization protocol |
Following fragmentation, 15 ug cRNA were hybridized for 16 h at 45°C on GeneChip HG-U219 or GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
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Description |
isolated from the vein of the umbilical cord
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Data processing |
The data were analyzed using Affymetrix Expression Console with RMA as normalization method
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Submission date |
Oct 16, 2018 |
Last update date |
Mar 04, 2019 |
Contact name |
Jakob Admard |
Organization name |
Universitätsklinikum Tübingen
|
Department |
Institut für Medizinische Genetik und Angewandte Genomik
|
Street address |
Calwerstr. 7
|
City |
Tübingen |
ZIP/Postal code |
72076 |
Country |
Germany |
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|
Platform ID |
GPL570 |
Series (1) |
GSE121297 |
Rigidity and inflammatory responses of interconnected endothelial cells are stimulated by rhodocetin-αβ via the neuropilin-1-MET-axis |
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