|
| Status |
Public on Mar 04, 2019 |
| Title |
E13R040b02: B2 (Bolus200nM_RCab) |
| Sample type |
RNA |
| |
|
| Source name |
Human umbilical vein endothelial cells, RCαβ treated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human umbilical vein endothelial cells
passage: passage 4
|
| Treatment protocol |
Endothelial cells stimulated with 200 nM RCαβ or 200 ng/ml HGF
|
| Growth protocol |
Human umbilical vein endothelial cells (HUVECs) were cultured according to the manufacturer’s instructions (Promocell, passages < 5), in complete endothelial cell growth medium containing 2% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected and total RNA was extracted from the cells.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard GeneChip WT Kit protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug cRNA were hybridized for 16 h at 45°C on GeneChip HG-U219 or GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
isolated from the vein of the umbilical cord (promocell)
|
| Data processing |
The data were analyzed using Affymetrix Expression Console with RMA as normalization method
|
| |
|
| Submission date |
Oct 16, 2018 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Jakob Admard |
| Organization name |
Universitätsklinikum Tübingen
|
| Department |
Institut für Medizinische Genetik und Angewandte Genomik
|
| Street address |
Calwerstr. 7
|
| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE121297 |
Rigidity and inflammatory responses of interconnected endothelial cells are stimulated by rhodocetin-αβ via the neuropilin-1-MET-axis |
|
