|
| Status |
Public on Mar 01, 2024 |
| Title |
normal diet [CvsQ], biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
rat fed a normal diet for 8 weeks
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
tissue: blood
diet: normal
|
| Treatment protocol |
Blood was collected, homogenated with TRIzol LS reagent (Invitrogen Life Technologies, Carlsbad, CA, USA), and stored at -80˚C
|
| Growth protocol |
Male Wistar rats (7 weeks old) were purchased from Japan SLC, Inc. (Shizuoka, Japan) and housed in a room that was maintained at 21 ± 1°C and 50 ± 10% relative humidity with a 12-h light/dark cycle (light 08:00–20:00; dark 20:00–08:00). The rats were preliminary given a normal diet (CE-2, CLEA Japan, Kanagawa, Japan) for acclimation. After a week of acclimation, the rats were divided into three groups (n=10 each) and fed for 8 weeks on a high-fat diet (Quick fat, CLEA Japan, Kanagawa, Japan), a high-fat diet plus 1% mulberry leaf powder, or a normal diet. Mulberry leaf powder was obtained from Sagamihara chamber of commerce and industry (Kanagawa, Japan). The rats were given a specific diet and water for 24 h ad libitum. On day 56 after overnight fasting, the animals were euthanized under anesthesia to collect their blood.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol LS reagent (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA (GeneChip® 3' IVT Express kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip® Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
no additional information
|
| Data processing |
The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the DFW.
|
| |
|
| Submission date |
Mar 26, 2018 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Asuka Kamei |
| E-mail(s) |
fp-kamei@newkast.or.jp
|
| Phone |
+81-44-280-2187
|
| Organization name |
Kanagawa Academy of Science and technology
|
| Department |
Project for development of food functionality assessment methods
|
| Street address |
LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
|
| City |
Kanagawa |
| ZIP/Postal code |
210-0821 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE112332 |
Effects of mulberry (Morus alba L.) leaf ingestion on gene expression of peripheral whole blood [CvsQ] |
| GSE112338 |
Effects of mulberry (Morus alba L.) leaf ingestion on gene expression of peripheral whole blood |
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