|
| Status |
Public on May 13, 2008 |
| Title |
C2C12 myotube differentiation Day 4 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 myotube differentiation Day 4
|
| Organism |
Mus musculus |
| Characteristics |
Age: C2C12 myotube differentiation Day 4
|
| Treatment protocol |
Differentiation of C2C12 myoblasts into myotubes was achieved by culturing cells in medium containing reduced serum concentration (2% v/v) for up to 5 days with medium changes every 2 days.
|
| Growth protocol |
C2C12 mouse skeletal myoblasts were cultured in DMEM high glucose with 10% FBS and 1% penicillin/streptomycin (Gibco) and maintained at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
C2C12 cell RNA was harvested using the RNeasy Midiprep kit (Qiagen) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated RNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
RNA were hybridized on Affymetrix mouse whole-genome microarray, MOE430 Plus 2.0 chips, Each time point was performed in triplicate, from independent experiments.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
C2C12 Differentiation timecourse to observe changes between myoblasts and myotubes
|
| Data processing |
The data were analyzed with Bioconductor GCRMA normalization method. The log-scaled data were unlogged to be comparable to MAS 5 raw value. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
May 12, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Qicheng Ma |
| Organization name |
Novartis
|
| Street address |
250 Mass Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11415 |
C2C12 differentiation time course to observe changes between myoblasts and myotubes |
|
| Relations |
| Reanalyzed by |
GSE119085 |
