|
| Status |
Public on Apr 12, 2008 |
| Title |
Hdh-KO-27 replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
MEF cells
|
| Organism |
Mus musculus |
| Characteristics |
Hdh-KO-27 replicate2
|
| Growth protocol |
MEF cells were cultured in 10% FBS DMEM medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA were extracted followed TRIZOL protocol, then transferred to RNAeasy mini spin columns for RNA purification
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion, Inc., Austin, TX) according to the manufacturer's directions starting with ~200 ng total RNA
|
| |
|
| Hybridization protocol |
performed according to the Illumina BeadStation 500× manual (revision C)
|
| Scan protocol |
Arrays were scanned with an Illumina Bead array Reader confocal scanner
|
| Description |
none
|
| Data processing |
the data was analyzed using Illumina's BeadStudio software(Version 3).
|
| |
|
| Submission date |
Apr 11, 2008 |
| Last update date |
Apr 11, 2008 |
| Contact name |
hua zhang |
| E-mail(s) |
hua.zhang@utsouthwestern.edu
|
| Phone |
2146456016
|
| Fax |
2146456018
|
| Organization name |
utsw
|
| Department |
physiology
|
| Street address |
5323 harry hines
|
| City |
dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6731 |
| Series (1) |
| GSE11139 |
Elucidating a normal function of huntingtin by analysis of huntingtin-null mouse embryonic fibroblasts |
|
