|
Status |
Public on Apr 12, 2008 |
Title |
Hdh-KO-16 replicate1 |
Sample type |
RNA |
|
|
Source name |
MEF cells
|
Organism |
Mus musculus |
Characteristics |
Hdh-KO-16 replicate1
|
Growth protocol |
MEF cells were cultured in 10% FBS DMEM medium
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA were extracted followed TRIZOL protocol, then transferred to RNAeasy mini spin columns for RNA purification
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion, Inc., Austin, TX) according to the manufacturer's directions starting with ~200 ng total RNA
|
|
|
Hybridization protocol |
performed according to the Illumina BeadStation 500× manual (revision C)
|
Scan protocol |
Arrays were scanned with an Illumina Bead array Reader confocal scanner
|
Description |
none
|
Data processing |
the data was analyzed using Illumina's BeadStudio software(Version 3).
|
|
|
Submission date |
Apr 11, 2008 |
Last update date |
Apr 11, 2008 |
Contact name |
hua zhang |
E-mail(s) |
hua.zhang@utsouthwestern.edu
|
Phone |
2146456016
|
Fax |
2146456018
|
Organization name |
utsw
|
Department |
physiology
|
Street address |
5323 harry hines
|
City |
dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL6731 |
Series (1) |
GSE11139 |
Elucidating a normal function of huntingtin by analysis of huntingtin-null mouse embryonic fibroblasts |
|