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Status |
Public on Apr 16, 2008 |
Title |
Hypothalamus; Large Wite; Female (HYPT_LW_F) |
Sample type |
RNA |
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|
Source name |
Hypothalamus; Large Wite; Female
|
Organism |
Sus scrofa |
Characteristics |
weight: 36.20 kg Age: 87 days
|
Treatment protocol |
At necropsy, samples of 16 tissues were collected, snap frozen in liquid nitrogen and stored at -80 ºC, until de RNA extraction.
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Growth protocol |
Pigs with one month of age were housed simultaneously, fed the same diets during the fattening period, that lasted two months.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from 100 mg tissue using the RiboPure™ kit (Ambion, Austin, USA) according to the manufacturer’s protocol.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
The hybridization was done according to Affymetrix standard protocols.
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Scan protocol |
Standard Affymetrix protocol
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Description |
Hypothalamus; Large Wite; Female
|
Data processing |
Microarray expression data were generated with GeneChip Operating Software (GCOS)
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|
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Submission date |
Mar 19, 2008 |
Last update date |
Apr 16, 2008 |
Contact name |
Miguel Perez-Enciso |
E-mail(s) |
miguel.perez@uab.es
|
Phone |
+34935814225
|
URL |
http://www.icrea.es
|
Organization name |
Universitat Autonoma Barcelona
|
Department |
Animal Science
|
Lab |
Animal Breeding
|
Street address |
Veterinary School
|
City |
Bellaterra |
ZIP/Postal code |
08193 |
Country |
Spain |
|
|
Platform ID |
GPL3533 |
Series (1) |
GSE10898 |
Transcriptome architecture across tissues in the pig |
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