|
| Status |
Public on Apr 16, 2008 |
| Title |
Hypothalamus; Large Wite; Male (HYPT_LW_M) |
| Sample type |
RNA |
| |
|
| Source name |
Hypothalamus; Large Wite; Male
|
| Organism |
Sus scrofa |
| Characteristics |
weight: 38.80 kg
Age: 87 days
|
| Treatment protocol |
At necropsy, samples of 16 tissues were collected, snap frozen in liquid nitrogen and stored at -80 ºC, until de RNA extraction.
|
| Growth protocol |
Pigs with one month of age were housed simultaneously, fed the same diets during the fattening period, that lasted two months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100 mg tissue using the RiboPure™ kit (Ambion, Austin, USA) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The hybridization was done according to Affymetrix standard protocols.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Hypothalamus; Large Wite; Male
|
| Data processing |
Microarray expression data were generated with GeneChip Operating Software (GCOS)
|
| |
|
| Submission date |
Mar 19, 2008 |
| Last update date |
Apr 16, 2008 |
| Contact name |
Miguel Perez-Enciso |
| E-mail(s) |
miguel.perez@uab.es
|
| Phone |
+34935814225
|
| URL |
http://www.icrea.es
|
| Organization name |
Universitat Autonoma Barcelona
|
| Department |
Animal Science
|
| Lab |
Animal Breeding
|
| Street address |
Veterinary School
|
| City |
Bellaterra |
| ZIP/Postal code |
08193 |
| Country |
Spain |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE10898 |
Transcriptome architecture across tissues in the pig |
|
