|
| Status |
Public on Jul 07, 2020 |
| Title |
SE-14000 stimulated Human Microvascular Endotheilal Cells vs. control 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human Microvascular Endotheilal Cells, 14000V/3days
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human Microvascular Endotheilal Cells
cell line: HMEC-1 (ATCCÂ CRL-3243)
|
| Treatment protocol |
cells were treated by 3hrs stimulation of 14000V and 90Hz for three days by SE-14000
|
| Growth protocol |
cells were kept in medium with 2% Fetal calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Mini Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Channel 2 |
| Source name |
Human Microvascular Endotheilal Cells, untreated control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human Microvascular Endotheilal Cells
cell line: HMEC-1 (ATCCÂ CRL-3243)
|
| Treatment protocol |
cells were treated by 3hrs stimulation of 14000V and 90Hz for three days by SE-14000
|
| Growth protocol |
cells were kept in medium with 2% Fetal calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Mini Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies SureScan G4900DA at 3 micron resolution. Images were quantified using Agilent Feature Extraction software version 11.5.1.1
|
| Description |
Biological replicate 1 of 3. Control Human Microvascular Endotheilal Cells, untreated, harvested after several passages.
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jul 07, 2017 |
| Last update date |
Jul 07, 2020 |
| Contact name |
noriko muto matsumoto |
| E-mail(s) |
nononoriko@nms.ac.jp
|
| Phone |
+81-3-3822-2131
|
| Organization name |
Nippon Medical School
|
| Department |
Department of Plastic, Reconstructive and Regenerative Surgery
|
| Street address |
1-1-5 Sendagi, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE100902 |
Wound healing related cells: Control vs. SE-14000 stimulation |
|
