|
| Status |
Public on Jun 01, 2018 |
| Title |
BE(2C).miR204 (+ dox) #1 |
| Sample type |
RNA |
| |
|
| Source name |
BE(2C).miR204 (+ dox)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: neuroblastoma
cell line: BE(2)C neuroblastoma cells with stably incorporated miR.204
treatment: +dox
genotype/variation: miR204 overexpression
|
| Treatment protocol |
Doxcycline (1mg/ml) was added to media to induce miR204 overexpression and was compared to a vehicle control (DMSO). RNA was isolated 48 hours after doxycycline was added to cells
|
| Growth protocol |
BE(2)C.miR204 cells subcultured every 3 days. Grown in DMEM 10%FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Purelink RNA kit (Life Technologies)
|
| Label |
Cy3
|
| Label protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
| |
|
| Hybridization protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
| Scan protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
| Data processing |
Microarrays and data were summarized and normalized with the vsn method in the R statistical programming language using the ‘limma’ package.
|
| |
|
| Submission date |
Jun 29, 2017 |
| Last update date |
Jun 01, 2018 |
| Contact name |
Daniel Carter |
| E-mail(s) |
dcarter@ccia.unsw.edu.au
|
| Organization name |
Children's Cancer Institute
|
| Street address |
Lowy Cancer Research Centre
|
| City |
Randwick |
| ZIP/Postal code |
2031 |
| Country |
Australia |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE100658 |
miR-204 blocks MYCN expression and is a novel a tumor suppressor in neuroblastoma tumorigenesis |
|
