|
Status |
Public on Jun 01, 2018 |
Title |
BE(2C).miR204 (no dox) #1 |
Sample type |
RNA |
|
|
Source name |
BE(2C).miR204 (no dox)
|
Organism |
Homo sapiens |
Characteristics |
tissue: neuroblastoma cell line: BE(2)C neuroblastoma cells with stably incorporated miR.204 treatment: no dox genotype/variation: control
|
Treatment protocol |
Doxcycline (1mg/ml) was added to media to induce miR204 overexpression and was compared to a vehicle control (DMSO). RNA was isolated 48 hours after doxycycline was added to cells
|
Growth protocol |
BE(2)C.miR204 cells subcultured every 3 days. Grown in DMEM 10%FCS
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the Purelink RNA kit (Life Technologies)
|
Label |
Cy3
|
Label protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
|
|
Hybridization protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
Scan protocol |
Performed by Ramaciotti Centre of Genomics, UNSW
|
Data processing |
Microarrays and data were summarized and normalized with the vsn method in the R statistical programming language using the ‘limma’ package.
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|
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Submission date |
Jun 29, 2017 |
Last update date |
Jun 01, 2018 |
Contact name |
Daniel Carter |
E-mail(s) |
dcarter@ccia.unsw.edu.au
|
Organization name |
Children's Cancer Institute
|
Street address |
Lowy Cancer Research Centre
|
City |
Randwick |
ZIP/Postal code |
2031 |
Country |
Australia |
|
|
Platform ID |
GPL21185 |
Series (1) |
GSE100658 |
miR-204 blocks MYCN expression and is a novel a tumor suppressor in neuroblastoma tumorigenesis |
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