|
| Status |
Public on Jan 25, 2018 |
| Title |
Mutu_59_EBVpos |
| Sample type |
RNA |
| |
|
| Source name |
Mutu_EBV positive
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Mutu
cell line clone id: Mutu_59
ebv status: EBV positive
|
| Treatment protocol |
None
|
| Growth protocol |
Cells were maintained in exponential growth in RPMI1640 medium containing 10% foetal calf serum and 2mM glutamine. Cells were maintained in exponential growth in RPMI1640 medium containing 10% foetal calf serum and 2mM glutamine. Cells were maintained in exponential growth in RPMI1640 medium containing 10% foetal calf serum and 2mM glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Trizol according to the manufacturer's instructions. RNA quality was confirmed with an Agilent 2100 BioAnalyser and all samples had RIN values >9.8.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
|
| |
|
| Hybridization protocol |
10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
|
| Scan protocol |
Affymetrix Standard protocol Affymetrix Standard protocol
|
| Description |
Gene expression data from EBV positive cell line
|
| Data processing |
Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project and HGU133Plus2_Hs_ENTREZG v21.0.0
log2 gene expression obtained by RMA method of the affy package in Bioconductor using default parameters
|
| |
|
| Submission date |
Jun 26, 2017 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Wenbin Wei |
| E-mail(s) |
Wenbin.Wei2@durham.ac.uk
|
| Organization name |
Durham University
|
| Department |
Biosciences
|
| Street address |
Stockton Road
|
| City |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23270 |
| Series (1) |
| GSE100458 |
Gene expression in EBV-positive versus EBV-loss clones derived from endemic Burkitt lymphoma cell lines |
|
