|
| Status |
Public on Mar 31, 2008 |
| Title |
Primary lung fibroblasts, RNA expression, technical replicate A |
| Sample type |
RNA |
| |
|
| Source name |
Primary lung fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
Total RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from 107-2x107 cells using
RNAeasy Kit (QIAGEN) following the manufacturer’s instructions. RNA quality was ensured sing the Agilent Systems Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated RNA was prepared with the Illumina TotalPrep RNA Amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
34ul of the Hyb mix with labeled cRNA sample was dispensed onto the center of each array and allowed to hyb with rotations in a pre-heated oven for 16-20h at 55*C. Washes were done with Wash E1BC solution, Block E1 solution was used for blocking and detection performed by Block E1 solution+Streptavidin-Cy3/5.
|
| Scan protocol |
Standard Illumina procedure
|
| Description |
no additional description
|
| Data processing |
Rank invariant normalization method implemented in Bead Studio software
|
| |
|
| Submission date |
Feb 11, 2008 |
| Last update date |
Mar 12, 2008 |
| Contact name |
Vitalina Komashko |
| E-mail(s) |
vitalina.komashko@sagebase.org
|
| Organization name |
Sage Bionetworks
|
| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6255 |
| Series (1) |
| GSE10504 |
Using ChIP-chip technology to reveal common principles of transcriptional repression in normal and cancer cells |
|
