|
| Status |
Public on May 18, 2017 |
| Title |
PE 40w |
| Sample type |
RNA |
| |
|
| Source name |
fibroblast, preeclampsia, 40w
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: placenta
cell type: fibroblast
gender: female
age: 33y
diagnosis: preeclampsia
gestational age (weeks): 40
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with the RNeasy Mini Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human Gene Expression Microarray 8x60K v3; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Description |
Gene expression
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. In addition, the raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
|
| |
|
| Submission date |
May 17, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Takako Ohmaru |
| E-mail(s) |
ohmaru2012@gmail.com
|
| Organization name |
Graduate School of Medical Sciences, Kyushu University
|
| Department |
Department of Gynecology and Obstetrics
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE99007 |
The difference of placental fibroblast between normal pregnancy and preeclampsia |
|
