|
| Status |
Public on Sep 18, 2017 |
| Title |
Rat_old_liver_2 |
| Sample type |
SRA |
| |
|
| Source name |
Rat_old_liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
Sex: male
age: 24 months
tissue: liver
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Purification of RNA from collected tissue samples was done using Qiagen RNeasy Mini Kit following the manufacturer's description
Library preparation was done using Illumina's TruSeq RNA Library Prep Kit v2 kit following the manufacturer's description.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
RatJOB2_Leber
|
| Data processing |
Extraction of read information in FastQ format was done using the tool bcl2astq v1.8.4 (provided by Illumina).
Reads were mapped using bwa aln (-n 2 -o 0 -e 0 -O 1000 -E 1000)
Only uniquely mapping reads were counted
Genome_build: RefSeq: mRNA sequences of Rattus norvegicus from 16.06.2014
Supplementary_files_format_and_content: The Excel sheet includes raw counts for all genes and each sample.
|
| |
|
| Submission date |
May 10, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Groth |
| E-mail(s) |
dnaseq@leibniz-fli.de
|
| Organization name |
Leibniz Institute on Aging - FLI
|
| Department |
Core Facility - Next Generation Sequencing
|
| Street address |
Beutenbergstraße 11
|
| City |
Jena |
| ZIP/Postal code |
07747 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18694 |
| Series (3) |
| GSE98745 |
RNA-seq of Wistar-rat liver from young and old animals |
| GSE98746 |
Aging in naked mole-rats and rats |
| GSE103066 |
RNA-seq of Wistar-rat liver and skin from young and old animals |
|
| Relations |
| BioSample |
SAMN06925360 |
| SRA |
SRX2793936 |
