|
| Status |
Public on Sep 15, 2017 |
| Title |
96hr siZNF148 Growth media 2 |
| Sample type |
RNA |
| |
|
| Source name |
Cell culture, LHCN-M2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Muscle
cell line: LHCN-M2
|
| Treatment protocol |
LHCN-M2 cells were treated with 20nM sicontrol or 20nM siZNF148 (dharmacon) for the indicated time in 6-well plates.
|
| Growth protocol |
LHCN-M2 cells were cultured in growth or differentiation media at 37°C, 5% CO2, as described by zhu et al, Aging Cell (2007) 6, pp515-523.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAGEN RNeasy Mini Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled (Cy3) according to Agilent's labeling protocol
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 65°C for 18 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer’s recommended protocols
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 3μm resolution, and the array data was extracted with Agilent feature extraction software using the GE1_107_Sep09 protocol
|
| Description |
Gene expression after 96 hours siZNF148 transfection confluent in growth media, biological replicate 2
|
| Data processing |
Agilent Feature Extraction Software (version 10.7.3.1) was used along with LOWESS normalization
|
| |
|
| Submission date |
Feb 02, 2017 |
| Last update date |
Sep 15, 2017 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE94441 |
Analysis of gene expression in human LHCN-M2 cells with siControl or siZNF 148 |
|
